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R&D Systems soluble cd163

Fig. 4 <t>sCD163</t> was significantly higher in IgAVN patients as com- pared to IgAV_GI patients. p-values were calculated using Mann– Whitney U test. Data are expressed as medians (Q25–Q75) of each group. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. IgAV, immunoglobulin A vasculitis; sl-IgAV, skin-limited IgAV; IgAVN, IgAV with renal involvement; IgAV_GI, IgA with gastrointestinal involvement (GI); IgAVN + GI, IgAV with GI and renal involvement; HC, healthy con- trols

Soluble Cd163, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+cd163/pm39953336-61-0-22?v=R%26D+Systems
Average 95 stars, based on 91 article reviews

soluble cd163 - by Bioz Stars, 2026-07

95/100 stars

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1) Product Images from "Haptoglobin as a novel predictor of visceral involvement and relapse in adult IgAV patients."

Article Title: Haptoglobin as a novel predictor of visceral involvement and relapse in adult IgAV patients.

Journal: Clinical rheumatology

doi: 10.1007/s10067-025-07363-6

Fig. 4 sCD163 was significantly higher in IgAVN patients as com- pared to IgAV_GI patients. p-values were calculated using Mann– Whitney U test. Data are expressed as medians (Q25–Q75) of each group. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. IgAV, immunoglobulin A vasculitis; sl-IgAV, skin-limited IgAV; IgAVN, IgAV with renal involvement; IgAV_GI, IgA with gastrointestinal involvement (GI); IgAVN + GI, IgAV with GI and renal involvement; HC, healthy con- trols

Figure Legend Snippet: Fig. 4 sCD163 was significantly higher in IgAVN patients as com- pared to IgAV_GI patients. p-values were calculated using Mann– Whitney U test. Data are expressed as medians (Q25–Q75) of each group. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. IgAV, immunoglobulin A vasculitis; sl-IgAV, skin-limited IgAV; IgAVN, IgAV with renal involvement; IgAV_GI, IgA with gastrointestinal involvement (GI); IgAVN + GI, IgAV with GI and renal involvement; HC, healthy con- trols

Techniques Used: MANN-WHITNEY

Image Search Results

Journal: Clinical rheumatology

Article Title: Haptoglobin as a novel predictor of visceral involvement and relapse in adult IgAV patients.

doi: 10.1007/s10067-025-07363-6

Figure Lengend Snippet: Fig. 4 sCD163 was significantly higher in IgAVN patients as com- pared to IgAV_GI patients. p-values were calculated using Mann– Whitney U test. Data are expressed as medians (Q25–Q75) of each group. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. IgAV, immunoglobulin A vasculitis; sl-IgAV, skin-limited IgAV; IgAVN, IgAV with renal involvement; IgAV_GI, IgA with gastrointestinal involvement (GI); IgAVN + GI, IgAV with GI and renal involvement; HC, healthy con- trols

Article Snippet: Soluble CD163 was measured in sera from 59 treatment-naïve adult IgAV patients and 22 age-/sex-matched HC using human CD163 Quantikine ELISA Kit (R&D Systems) according to manufacturer instructions.

Techniques: MANN-WHITNEY

Differential expression of IBA1 and CD163 in glioblastoma (adjacent tissue sections). Iba-1 labeling (A, C, E, G) is typically cellular whereas staining for CD163 (B, D, F, H) frequently shows extracellular deposition of the peroxidase reaction product, diaminobenzidine (DAB) (D, F, H) as well. In cases where we found differential expression, CD163 immunoreactivity could be very strong in some tissue areas where Iba-1 was weak in the corresponding part of the adjacent section (e.g., the area marked by the asterisk in C). The area in C shows a high density of nuclei probably representing tumor cells. However, other tissue areas (asterisk in D and corresponding area in C) could be almost completely devoid of staining. E and F represent another pair of adjacent tissue areas showing discrepant expression for the two microglia/macrophage markers, Iba-1 and CD163. In E, Iba-1 immunoreactivity is weak and very few recognizable microglia/macrophages are present. F demonstrates CD163 staining especially over tissue displaying reduced tissue integrity (necrosis?). In contrast, in H immunoreactivity for CD163 is much weaker than that of Iba-1 (G) in the corresponding tumor area (asterisk in H) but CD163 labeling is stronger than that for Iba-1 towards the upper tumor margin (necrosis?) Scale bar: 500 µm for A and B, 200 µm for C to H.

Journal: Oncology Research

Article Title: Microglia and brain macrophages are differentially associated with tumor necrosis in glioblastoma: A link to tumor progression

doi: 10.32604/or.2024.056436

Figure Lengend Snippet: Differential expression of IBA1 and CD163 in glioblastoma (adjacent tissue sections). Iba-1 labeling (A, C, E, G) is typically cellular whereas staining for CD163 (B, D, F, H) frequently shows extracellular deposition of the peroxidase reaction product, diaminobenzidine (DAB) (D, F, H) as well. In cases where we found differential expression, CD163 immunoreactivity could be very strong in some tissue areas where Iba-1 was weak in the corresponding part of the adjacent section (e.g., the area marked by the asterisk in C). The area in C shows a high density of nuclei probably representing tumor cells. However, other tissue areas (asterisk in D and corresponding area in C) could be almost completely devoid of staining. E and F represent another pair of adjacent tissue areas showing discrepant expression for the two microglia/macrophage markers, Iba-1 and CD163. In E, Iba-1 immunoreactivity is weak and very few recognizable microglia/macrophages are present. F demonstrates CD163 staining especially over tissue displaying reduced tissue integrity (necrosis?). In contrast, in H immunoreactivity for CD163 is much weaker than that of Iba-1 (G) in the corresponding tumor area (asterisk in H) but CD163 labeling is stronger than that for Iba-1 towards the upper tumor margin (necrosis?) Scale bar: 500 µm for A and B, 200 µm for C to H.

Article Snippet: Increased levels of soluble CD163 can be detected in biofluids, e.g., CSF (cerebrospinal fluid), and used to monitor the activation of macrophages.

Techniques: Quantitative Proteomics, Labeling, Staining, Expressing

Examples of tumor necrosis are shown in (A–F). Iba-1 labeling is illustrated in the left column, and CD163 is on the right. In A, palisading microglia/macrophages (arrows) are strongly labeled for Iba-1 while expression for CD163 in the corresponding adjacent tumor area is much weaker but discernible on some palisading cells (B). It is noteworthy that the hypocellular part of the necrosis is strongly CD163 positive (staining is largely extracellular). C, A rim of Iba-1 immunoreactivity consisting of palisading microglia/macrophages surrounds a necrotic tissue area (corresponding asterisk in D) which is immunonegative for Iba-1 (C) but marked strongly for CD163 (D). E and F again illustrate significant differences between Iba-1 and CD163 expression. Iba-1 is expressed mainly in the upper half of what likely represents a cortical gyrus that is infiltrated by tumor cells. Palisading Iba-1 positive microglia/macrophages (cf. A and B) form a demarcation line (“rim”, arrows) above the necrotic tissue area which is largely devoid of Iba-1 staining but shows strong CD163 immunoreactivity in F. There is some accentuated CD163 staining at the rim. However, CD163 labeling is largely diffuse and most pronounced in the hypocellular tissue area (F). Scale bar: 200 µm in A, B; 1 mm in C-F. A and B are taken from Case 25, and are taken from Case 1, and E and F are taken from Case 6. N, necrotic core.

Journal: Oncology Research

Article Title: Microglia and brain macrophages are differentially associated with tumor necrosis in glioblastoma: A link to tumor progression

doi: 10.32604/or.2024.056436

Figure Lengend Snippet: Examples of tumor necrosis are shown in (A–F). Iba-1 labeling is illustrated in the left column, and CD163 is on the right. In A, palisading microglia/macrophages (arrows) are strongly labeled for Iba-1 while expression for CD163 in the corresponding adjacent tumor area is much weaker but discernible on some palisading cells (B). It is noteworthy that the hypocellular part of the necrosis is strongly CD163 positive (staining is largely extracellular). C, A rim of Iba-1 immunoreactivity consisting of palisading microglia/macrophages surrounds a necrotic tissue area (corresponding asterisk in D) which is immunonegative for Iba-1 (C) but marked strongly for CD163 (D). E and F again illustrate significant differences between Iba-1 and CD163 expression. Iba-1 is expressed mainly in the upper half of what likely represents a cortical gyrus that is infiltrated by tumor cells. Palisading Iba-1 positive microglia/macrophages (cf. A and B) form a demarcation line (“rim”, arrows) above the necrotic tissue area which is largely devoid of Iba-1 staining but shows strong CD163 immunoreactivity in F. There is some accentuated CD163 staining at the rim. However, CD163 labeling is largely diffuse and most pronounced in the hypocellular tissue area (F). Scale bar: 200 µm in A, B; 1 mm in C-F. A and B are taken from Case 25, and are taken from Case 1, and E and F are taken from Case 6. N, necrotic core.

Article Snippet: Increased levels of soluble CD163 can be detected in biofluids, e.g., CSF (cerebrospinal fluid), and used to monitor the activation of macrophages.

Techniques: Labeling, Expressing, Staining

Representative microglia and macrophage morphologies in glioblastoma. (A) Ramified microglia showing expression of IBA1. (B) Strongly activated microglia display more intensely stained, stouter cell processes. (C) Rounder, macrophage-like microglia with strong IBA1 expression that are largely devoid of processes. (D) Isolated remnants of microglial cells with residual IBA1 immunoreactivity in a necrotic tumor area. (E) Perivascular extra- and intraparenchymal macrophages which are strongly CD163 positive. The distribution shown is reminiscent of recent infiltration from the bloodstream as seen in experimental animal studies. (F) Strong and largely diffuse immunoreactivity for CD163 in a necrotic tumor area: the majority of the staining is found extracellularly. (G) CD163 expressing macrophages. (H) A microglial cell expressing CD163. Scale bar: 12 µm for A–D and G–H and 40 µm for E–F.

Journal: Oncology Research

Article Title: Microglia and brain macrophages are differentially associated with tumor necrosis in glioblastoma: A link to tumor progression

doi: 10.32604/or.2024.056436

Figure Lengend Snippet: Representative microglia and macrophage morphologies in glioblastoma. (A) Ramified microglia showing expression of IBA1. (B) Strongly activated microglia display more intensely stained, stouter cell processes. (C) Rounder, macrophage-like microglia with strong IBA1 expression that are largely devoid of processes. (D) Isolated remnants of microglial cells with residual IBA1 immunoreactivity in a necrotic tumor area. (E) Perivascular extra- and intraparenchymal macrophages which are strongly CD163 positive. The distribution shown is reminiscent of recent infiltration from the bloodstream as seen in experimental animal studies. (F) Strong and largely diffuse immunoreactivity for CD163 in a necrotic tumor area: the majority of the staining is found extracellularly. (G) CD163 expressing macrophages. (H) A microglial cell expressing CD163. Scale bar: 12 µm for A–D and G–H and 40 µm for E–F.

Article Snippet: Increased levels of soluble CD163 can be detected in biofluids, e.g., CSF (cerebrospinal fluid), and used to monitor the activation of macrophages.

Techniques: Expressing, Staining, Isolation

WSI fusion heatmaps (C, F, I) demonstrating the differential expression of IBA1 (A, D, G) and CD163 (B, E, H). Areas of overlap are shown in red and discrepant areas in white in the fusion heat maps. There are large areas of CD163 immunoreactivity which is absent from healthy brain parenchyma. The expression of both markers was clearly divergent within and between cases as shown: Cases 32 (A–C), 5 (G–I), and 23 (D–F).

Journal: Oncology Research

Article Title: Microglia and brain macrophages are differentially associated with tumor necrosis in glioblastoma: A link to tumor progression

doi: 10.32604/or.2024.056436

Figure Lengend Snippet: WSI fusion heatmaps (C, F, I) demonstrating the differential expression of IBA1 (A, D, G) and CD163 (B, E, H). Areas of overlap are shown in red and discrepant areas in white in the fusion heat maps. There are large areas of CD163 immunoreactivity which is absent from healthy brain parenchyma. The expression of both markers was clearly divergent within and between cases as shown: Cases 32 (A–C), 5 (G–I), and 23 (D–F).

Article Snippet: Increased levels of soluble CD163 can be detected in biofluids, e.g., CSF (cerebrospinal fluid), and used to monitor the activation of macrophages.

Techniques: Quantitative Proteomics, Expressing

Key microglia/macrophage molecules and their interactions are schematically illustrated in relation to a typical glioblastoma necrosis. IBA1 (AIF1) and CD163 are the cellular microglia/brain macrophage markers used in this study. ADAM17 is responsible for inducing the ectodomain shedding of scavenger receptor CD163 from the membrane of activated macrophages. IL-6 is released into the hypoxic niche. IL-6 signaling may stimulate the expression of NT5E (CD73). Significant upregulation of CD44 is found in palisades of glioblastoma cells and nearby areas of necrosis. ADAM17 may also promote the cleavage of intracellular CD44. SPP1 (OPN) is a ligand of CD44 that is secreted by both myeloid and glioblastoma cells. “Created with BioRender.com (accessed on 12 November 2024)”.

Journal: Oncology Research

Article Title: Microglia and brain macrophages are differentially associated with tumor necrosis in glioblastoma: A link to tumor progression

doi: 10.32604/or.2024.056436

Figure Lengend Snippet: Key microglia/macrophage molecules and their interactions are schematically illustrated in relation to a typical glioblastoma necrosis. IBA1 (AIF1) and CD163 are the cellular microglia/brain macrophage markers used in this study. ADAM17 is responsible for inducing the ectodomain shedding of scavenger receptor CD163 from the membrane of activated macrophages. IL-6 is released into the hypoxic niche. IL-6 signaling may stimulate the expression of NT5E (CD73). Significant upregulation of CD44 is found in palisades of glioblastoma cells and nearby areas of necrosis. ADAM17 may also promote the cleavage of intracellular CD44. SPP1 (OPN) is a ligand of CD44 that is secreted by both myeloid and glioblastoma cells. “Created with BioRender.com (accessed on 12 November 2024)”.

Article Snippet: Increased levels of soluble CD163 can be detected in biofluids, e.g., CSF (cerebrospinal fluid), and used to monitor the activation of macrophages.

Techniques: Membrane, Expressing

Survival analysis in relation to CD163 expression (with sex serving as a control variable). As anticipated, male GBM patients demonstrated shorter survival in both cohorts. A mild negative association between increased CD163 expression and survival was observed in the second cohort, which is discussed further in the text.

Journal: Oncology Research

Article Title: Microglia and brain macrophages are differentially associated with tumor necrosis in glioblastoma: A link to tumor progression

doi: 10.32604/or.2024.056436

Figure Lengend Snippet: Survival analysis in relation to CD163 expression (with sex serving as a control variable). As anticipated, male GBM patients demonstrated shorter survival in both cohorts. A mild negative association between increased CD163 expression and survival was observed in the second cohort, which is discussed further in the text.

Article Snippet: Increased levels of soluble CD163 can be detected in biofluids, e.g., CSF (cerebrospinal fluid), and used to monitor the activation of macrophages.

Techniques: Expressing, Control

Genes sorted by support vector machine-recursive feature elimination (SVM-RFE) method.

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Genes sorted by support vector machine-recursive feature elimination (SVM-RFE) method.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Plasmid Preparation

Validation and receiver operating characteristic (ROC) curve analyses of CCR1 and CD163 in heart failure (HF) and nonalcoholic fatty liver disease (NAFLD). (A–D) Expression of CCR1 and CD163 in GSE26887 (A) , GSE57345 (B) , GSE126848 (C) , and GSE89632 (D) , respectively. (E–H) ROC curve and area under the curve (AUC) of CCR1 and CD163 in GSE26887 (E) , GSE57345 (F) , GSE126848 (G) , and GSE89632 (H) , respectively. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. healthy controls.

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Validation and receiver operating characteristic (ROC) curve analyses of CCR1 and CD163 in heart failure (HF) and nonalcoholic fatty liver disease (NAFLD). (A–D) Expression of CCR1 and CD163 in GSE26887 (A) , GSE57345 (B) , GSE126848 (C) , and GSE89632 (D) , respectively. (E–H) ROC curve and area under the curve (AUC) of CCR1 and CD163 in GSE26887 (E) , GSE57345 (F) , GSE126848 (G) , and GSE89632 (H) , respectively. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. healthy controls.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Expressing

Gene Set Enrichment Analysis (GSEA) of CCR1 and CD163 in GSE126848 and GSE26887 datasets. (A, B) Biological processes (A) and KEGG pathways (B) found by singe-gene GSEA of CCR1 in NAFLD dataset GSE126848. (C, D) Biological processes (C) and KEGG pathways (D) found by singe-gene GSEA of CD163 in NAFLD dataset GSE126848. (E, F) Biological processes (E) and KEGG pathways (F) found by singe-gene GSEA of CCR1 in HF dataset GSE26887. (G, H) Biological processes (G) and KEGG pathways (H) found by singe-gene GSEA of CD163 in HF dataset GSE26887.

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Gene Set Enrichment Analysis (GSEA) of CCR1 and CD163 in GSE126848 and GSE26887 datasets. (A, B) Biological processes (A) and KEGG pathways (B) found by singe-gene GSEA of CCR1 in NAFLD dataset GSE126848. (C, D) Biological processes (C) and KEGG pathways (D) found by singe-gene GSEA of CD163 in NAFLD dataset GSE126848. (E, F) Biological processes (E) and KEGG pathways (F) found by singe-gene GSEA of CCR1 in HF dataset GSE26887. (G, H) Biological processes (G) and KEGG pathways (H) found by singe-gene GSEA of CD163 in HF dataset GSE26887.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques:

Interaction of CCR1 and CD163 with inflammatory genes and chemical drugs. (A) Regulatory network of CCR1 and CD163 and their co-expression genes constructed by the GeneMANIA database. (B, C) The correlation between CCR1 and CD163 with inflammation-related genes in the GSE126848 (B) and GSE26887 (C) datasets. (D) The interactions between CCR1 and CD163 with chemicals and proteins constructed by the STITCH database.

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Interaction of CCR1 and CD163 with inflammatory genes and chemical drugs. (A) Regulatory network of CCR1 and CD163 and their co-expression genes constructed by the GeneMANIA database. (B, C) The correlation between CCR1 and CD163 with inflammation-related genes in the GSE126848 (B) and GSE26887 (C) datasets. (D) The interactions between CCR1 and CD163 with chemicals and proteins constructed by the STITCH database.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Expressing, Construct

Validation of CCR1 and CD163 in a non-alcoholic fatty liver disease mouse model. (A) Hematoxylin&eosin (H&E) staining of liver tissues in mice with normal chow (10% of calorie from fat, NC) or high-fat diet (60% of calorie from fat, HFD) for 14 weeks. The black arrow indicates infiltrated immune cells. Scale bar = 100 μm. (B) NAFLD activity score (NAS) based on the H&E staining of liver tissues. (C) Hepatic triglyceride (TG) concentrations. (D) Serum alanine aminotransferase (ALT) levels. (E) Relative mRNA expression level of inflammatory marker genes in liver tissues. (F–I) Relative mRNA expression level of Ccr1 (F) , Cd163 (G) , Cd80 (H) , and Cd206 (I) in liver tissues. (J) Representative images under fluorescence microscopy showing CD163 staining (red) and nuclear staining (DIPA, blue) of liver tissues. Scale bar = 100 μm. (K) Representative images under fluorescence microscopy showing CD80 staining (red) and nuclear staining (DIPA, blue) of liver tissues. Scale bar = 100 μm. Mean ± S.E.M., n = 12. * P< 0.05, ** P< 0.01 vs. the NC group.

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Validation of CCR1 and CD163 in a non-alcoholic fatty liver disease mouse model. (A) Hematoxylin&eosin (H&E) staining of liver tissues in mice with normal chow (10% of calorie from fat, NC) or high-fat diet (60% of calorie from fat, HFD) for 14 weeks. The black arrow indicates infiltrated immune cells. Scale bar = 100 μm. (B) NAFLD activity score (NAS) based on the H&E staining of liver tissues. (C) Hepatic triglyceride (TG) concentrations. (D) Serum alanine aminotransferase (ALT) levels. (E) Relative mRNA expression level of inflammatory marker genes in liver tissues. (F–I) Relative mRNA expression level of Ccr1 (F) , Cd163 (G) , Cd80 (H) , and Cd206 (I) in liver tissues. (J) Representative images under fluorescence microscopy showing CD163 staining (red) and nuclear staining (DIPA, blue) of liver tissues. Scale bar = 100 μm. (K) Representative images under fluorescence microscopy showing CD80 staining (red) and nuclear staining (DIPA, blue) of liver tissues. Scale bar = 100 μm. Mean ± S.E.M., n = 12. * P< 0.05, ** P< 0.01 vs. the NC group.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Activity Assay, Expressing, Marker, Fluorescence, Microscopy

$Validation of CCR1 and CD163 in a heart failure with reduced ejection fraction (HFpEF) mouse model. (A) Wheat germ agglutinin (WGA, green) staining of heart tissues in mice with saline (CON group) or uninephrectomy surgery followed by 0.15mg/h d-aldosterone (HFpEF group) treatment for 4 weeks. Scale bar = 100 μm. (B) Quantitative results of the left ventricular cross-sectional area based on the WGA staining of heart tissues. (C) Heart weight/body weight (HW/BW) ratio. (D–F) Relative mRNA expression level of left ventricular hypertrophy markers Anp (D) , Bnp (E) , and β-MHC (F) in heart tissues. (G) Relative mRNA expression level of inflammatory marker genes in heart tissues. (H–K) Relative mRNA expression level of Ccr1 (H) , Cd163 (I) , Cd80 (J) , and Cd206 (K) in heart tissues. (L) Representative images under fluorescence microscopy showing CD163 staining (red) and nuclear staining (DIPA, blue) of heart tissues. Scale bar = 100 μm. (M) Representative images under fluorescence microscopy showing CD80 staining (red) and nuclear staining (DIPA, blue) of heart tissues. Scale bar = 100 μm. Mean ± S.E.M., n = 6. * P< 0.05, ** P< 0.01 vs. the control group.$

Journal: Frontiers in Immunology

Article Title: Identification of common signature genes and pathways underlying the pathogenesis association between nonalcoholic fatty liver disease and heart failure

doi: 10.3389/fimmu.2024.1424308

Figure Lengend Snippet: Validation of CCR1 and CD163 in a heart failure with reduced ejection fraction (HFpEF) mouse model. (A) Wheat germ agglutinin (WGA, green) staining of heart tissues in mice with saline (CON group) or uninephrectomy surgery followed by 0.15mg/h d-aldosterone (HFpEF group) treatment for 4 weeks. Scale bar = 100 μm. (B) Quantitative results of the left ventricular cross-sectional area based on the WGA staining of heart tissues. (C) Heart weight/body weight (HW/BW) ratio. (D–F) Relative mRNA expression level of left ventricular hypertrophy markers Anp (D) , Bnp (E) , and β-MHC (F) in heart tissues. (G) Relative mRNA expression level of inflammatory marker genes in heart tissues. (H–K) Relative mRNA expression level of Ccr1 (H) , Cd163 (I) , Cd80 (J) , and Cd206 (K) in heart tissues. (L) Representative images under fluorescence microscopy showing CD163 staining (red) and nuclear staining (DIPA, blue) of heart tissues. Scale bar = 100 μm. (M) Representative images under fluorescence microscopy showing CD80 staining (red) and nuclear staining (DIPA, blue) of heart tissues. Scale bar = 100 μm. Mean ± S.E.M., n = 6. * P< 0.05, ** P< 0.01 vs. the control group.

Article Snippet: Serum soluble CD163 (sCD163) levels were detected using a commercial kit (EM1475; Finetest, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Saline, Expressing, Marker, Fluorescence, Microscopy, Control

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1) Product Images from "Haptoglobin as a novel predictor of visceral involvement and relapse in adult IgAV patients."

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